Central and Peripheral Effects of Lipopolysaccharide on Food Choice and Macronutrient Selection in Meat-Type Chick.

This report aimed to determine the effect of lipopolysaccharide (LPS) on food intake in broiler chicks with different rations. All birds received a starter diet until five days of age, but experimental diets were provided on days of injections. In experimental group one, chickens received an intracerebroventricular (ICV) injection of LPS (25, 50, and 100 ng) with a standard diet. In experimental group two, chickens received intraperitoneal (IP) injections of LPS (50, 100, and 200 µg) with a standard diet. In experimental group three, birds received ICV injections of saline and different diets. Accordingly, a standard diet without fat, a diet containing 20% higher nutrient energy than the standard, a diet containing 20% less nutrient energy than the standard, and a standard diet containing fat were offered to them to investigate the desire of chickens for the diets. Experimental groups four, five, and six were similar to experimental group three, except that the chickens received ICV injections of LPS. In experimental groups seven, eight, and nine, chickens received IP injections of LPS with different diets. Afterward, their cumulative food intake was measured until 180 min post-injection. According to the results, ICV and IP injections of LPS decreased food intake (P<0.05). However, the ICV injection of saline increased the desire of chickens for the standard diet with fat (P<0.05). The ICV injection of the LPS (50 and 100 ng) increased the appetite for a standard diet with nutrient energy 20% higher than the standard and a standard diet containing fat, at 120 and 180 min after the injection (P<0.05). In addition, IP injection of LPS (200 µg) significantly increased the desire for a standard diet with nutrient energy 20% higher than the standard and a standard diet containing fat (P<0.05). These results suggested the desire of chickens for different types of rations is affected by central or peripheral administration of the LPS.

Classifying subclinical depression using EEG spectral and connectivity measures.

Detecting depression on its early stages helps preventing the onset of severe depressive episodes. In this study, we propose an automatic classification pipeline to detect subclinical depression (i.e., dysphoria) through the electroencephalography (EEG) signal. To this aim, we recorded the EEG signals in resting condition from 26 female participants with dysphoria and 38 female controls. The EEG signals were processed to extract several spectral and functional connectivity features to feed a nonlinear Support Vector Machine (SVM) classifier embedded with a Recursive Feature Elimination (RFE) algorithm. Our recognition pipeline obtained a maximum classification accuracy of 83.91% in recognizing dysphoria patients with a combination of connectivity and spectral measures. Moreover, an accuracy of 76.11% was achieved with only the 4 most informative functional connections, suggesting a central role of cortical connectivity in the theta band for early depression recognition. The present study can facilitate the diagnosis of subclinical conditions of depression and may provide reliable indicators of depression for the clinical community.

Gaussian Processes with Physiologically-Inspired Priors for Physical Arousal Recognition.

While machine learning algorithms are able to detect subtle patterns of interest in data, expert knowledge may contain crucial information that is not easily extracted from a given dataset, especially when the latter is small or noisy. In this paper we investigate the suitability of Gaussian Process Classification (GPC) as an effective model to implement the domain knowledge in an algorithm's training phase. Building on their Bayesian nature, we proceed by injecting problem- specific domain knowledge in the form of an a-priori distribution on the GPC latent function. We do this by extracting handcrafted features from the input data, and correlating them to the logits of the classification problem through fitting a prior function informed by the physiology of the problem. The physiologically-informed prior of the GPC is then updated through the Bayes formula using the available dataset. We apply the methods discussed here to a two-class classification problem associated to a dataset comprising Heart Rate Variability (HRV) and Electrodermal Activity (EDA) signals collected from 26 subjects who were exposed to a physical stressor aimed at altering their autonomic nervous systems dynamics. We provide comparative computational experiments on the selection of appropriate physiologically-inspired GPC prior functions. We find that the recognition of the presence of the physical stressor is significantly enhanced when the physiologically-inspired prior knowledge is injected into the GPC model.

Quantification of Different Regulatory Pathways Contributing to Heartbeat Dynamics during Multiple Stimuli: a Proof of the Concept.

The dynamical interplay between brain and heart is mediated by several feedback mechanisms including the central autonomic network and baroreflex loop at a peripheral level, also for a short-term regulation. State of the art focused on the characterization of each regulatory pathway through a single stressor elicitation. However, no studies targeted the actual quantification of different mediating routes leading to the generation of heartbeat dynamics, particularly in case of combined exogenous stimuli. In this study, we propose a new approach based on computational modeling to quantify the contribution of multiple concurrent stimuli in modulating cardiovascular dynamics. In this preliminary attempt, the model estimates the high-frequency power of heartbeat dynamics, and derives disentangling coefficients quantifying the effect of multiple elicitations. Model evaluation is performed on healthy rate variability (HRV) series from fourteen healthy subjects undergoing physical (tilt-table) and mental stressors (aritmetics), as well as their combined administration. Results indicate that, at a group-wise level, in base of concurrent physical and mental elicitations, the physical stressor contributes for the 85% of the resulting heartbeat dynamics. These findings are in agreement with the current knowledge on heartbeat regulatory systems, providing valuable perspectives on the quantification of underlying generative mechanisms of HRV.

A New Sympathovagal Balance Index from Electrodermal Activity and Instantaneous Vagal Dynamics: A Preliminary Cold Pressor Study.

Sympathovagal balance, an autonomic index resulting from the sympathetic and parasympathetic influences on cardiovascular control, has been extensively used in the research practice. The current assessment is based on analyzing Heart Rate Variability (HRV) series in the frequency domain by regarding the ratio between the low and high frequency components (LF/HF). Nevertheless, LF and HF powers are known to be both influenced by vagal activity which strongly bias the accuracy of this method. To this extent, in this study we combine time-varying estimates from electrodermal activity (EDA) and HRV to propose a novel index of sympathovagal balance. Particularly, sympathetic activity is estimated from the EDA power calculated within the 0.045-0.25Hz bandwidth $(EDA_{Symp})$, whereas parasympathetic dynamics is measured instantaneously through a point-process modeling framework devised for heartbeat dynamics $(HF_{pp})$. We test our new index $SV = EDA_{Symp/HF_{pp}}$ on data gathered from 22 healthy subjects (7 females and 15 males) undergoing a 3 minutes gold standard protocol for sympathetic elicitation as the cold-pressor test (CPT). Results show that the activation of the proposed sympathovagal tone is consistent with CPT elicitation and is associated with a significantly higher statistical discriminant power than the standard LF/HF ratio, also revealing different dynamics between female and male subjects.

A new Modelling Framework to Study Time-Varying Directional Brain-Heart Interactions: Preliminary Evaluations and Perspectives.

We propose a novel modelling framework to study non-stationary, directional brain-heart interplay in a time varying fashion. Considering electroencephalographic (EEG) signals and Heart Rate Variability (HRV) series as inputs, a new multivariate formulation is derived from proper coupling functions linking cortical electrical activity and heartbeat dynamics generation models. These neural-autonomic coupling rules are formalised according to the current knowledge on the central autonomic network and fully parametrised in adaptive coefficients quantifying the information outflow from-brain-to- heart as well as from-heart-to-brain. Such coefficients can be effectively estimated by solving the model inverse problem, and profitably exploited for a novel assessment of brain-heart interactions. Here we show preliminary experimental results gathered from 27 healthy volunteers undergoing significant sympatho-vagal perturbations through cold-pressor test and discuss prospective uses of this novel methodological frame- work. Specifically, we highlight how the directional brain-heart coupling significantly increases during prolonged baroreflex elicitation with specific time delays and throughout specific brain areas, especially including fronto-parietal regions and lateralisation mechanisms in the temporal cortices.

Epidemiology and histopathological features of esophageal cancer.

INTRODUCTION: Esophageal cancer is the 6th most common cause of cancer death in the world. During the past two decades some changes have occurred in histologic type and primary tumor location in some parts of the world but there is a little information about histopathological aspect of disease in Iran. The purpose of the present study was to clarify the features of histopathological of esophageal cancer in Iran. METHODS: This study designed as a cross sectional study. Data analysis was performed with descriptive and univariate methods. Information required for this study extracted from cancer registry forms. The cases were all patients who received a histopathological diagnosis of EC that were referred to this center from January 2003 to October 2008. RESULTS: A total of 264 EC cases were recruited in the study the lower third of the esophagus was the most frequent site of origin of these tumors. Squamus cell carcinoma was commonest histologic type of tumor. In 15.2% of cases tumor was metastasis to other organs. In 46.6% were in advanced stage at diagnosis. DISCUSSION: Based on result of present study, esophageal cancer in our country more presented in Squamus cell carcinoma histology type, in advanced stage. Since Squamus cell carcinoma have a poorer prognosis and given that these patients have vague symptomatology for approximately several months, therefore, early diagnosis and treatment led to better prognosis.

Carcass performance and meat mineral content in Balouchi lamb fed pistachio by-products.

This experiment investigated the effect of pistachio by-products (PB) in the feed of weaned Balouchi lambs on their daily gain, carcass traits and on the levels of Ca, Zn, Fe and Cu of Longissimus dorsi muscle. Twenty eight male lambs were divided into four groups with 7 lambs each and fed separately for 90days. The first group (control) was fed a commercial concentrate, and the second, third and fourth (test) groups received diets in which 10, 20 and 30% PB was used in partial replacement of alfalfa hay and beet pulp. The results showed that the high level of PB had no effect on performance and carcass traits. There were no significant differences between groups in mineral content in Longissimus dorsi muscle. These results suggested that PB can be use in diet of fattening lambs up to 30% without any adverse effects on carcass performance and mineral content of lamb meat.

Detection of West Nile virus genome and specific antibodies in Iranian encephalitis patients.

West Nile virus (WNV) is a mosquito-borne flavivirus which circulates in birds, horses and humans. An estimated 80% of WNV infections are asymptomatic. Fewer than 1% of infected persons develop neuroinvasive disease, which typically presents as encephalitis, meningitis, or acute flaccid paralysis. This study was conducted from January 2008 to June 2009 in Isfahan, Iran. Patients attending the emergency department with fever and loss of consciousness were consecutively included. Cerebrospinal fluids (CSF) were initially analysed through bacteriology and biochemistry examinations, resulting in those with evidence of meningitis being excluded. Patients' CSF and serum were diagnosed by serological and molecular assays. A total of 632 patients with fever and loss of consciousness were tested by CSF analyses. Samples of the remaining patients (39·4%) were referred for WNV investigation. Three (1·2%) of the patients were positive for both serum and CSF by RT-PCR, and six (2·4%) were positive only for IgG antibodies. History of insect bite, and blood transfusion and transplantation were risk factors for being positive by RT-PCR (P=0·048) and being IgG positive (P=0·024), respectively. The results of this study showed that the prevalence of West Nile fever is low in patients with encephalitis.

Mice orally immunized with a transgenic plant expressing the glycoprotein of Crimean-Congo hemorrhagic fever virus.

While Crimean-Congo hemorrhagic fever (CCHF) has a high mortality rate in humans, the associated virus (CCHFV) does not induce clinical symptoms in animals, but animals play an important role in disease transmission to humans. Our aim in this study was to examine the immunogenicity of the CCHFV glycoprotein when expressed in the root and leaf of transgenic plants via hairy roots and stable transformation of tobacco plants, respectively. After confirmatory analyses of transgenic plant lines and quantification of the expressed glycoprotein, mice were either fed with the transgenic leaves or roots, fed the transgenic plant material and injected subcutaneously with the plant-made CCHFV glycoprotein (fed/boosted), vaccinated with an attenuated CCHF vaccine (positive control), or received no treatment (negative control). All immunized groups had a consistent rise in anti-glycoprotein IgG and IgA antibodies in their serum and feces, respectively. The mice in the fed/boosted group showed a significant rise in specific IgG antibodies after a single boost. Our results imply that oral immunization of animals with edible materials from transgenic plants is feasible, and further assessments are under way. In addition, while the study of CCHF is challenging, our protocol should be further used to study CCHFV infection in the knockout mouse model and virus neutralization assays in biosafety level 4 laboratories.

Molecular epidemiology of Crimean- Congo hemorrhagic fever virus genome isolated from ticks of Hamadan province of Iran.

BACKGROUND & OBJECTIVES: Crimean-Congo hemorrhagic fever (CCHF) virus is a tick-borne member of the genus Nairovirus, family Bunyaviridae. CCHFV has been isolated from at least 31 different tick species. The virus is transmitted through the bite of an infected tick, or by direct contact with CCHFV-infected patients or the products of infected livestock. This study was undertaken to study the genetic relationship and distribution of CCHFV in the tick population of Hamadan province of Iran. METHOD: In this study, RT-PCR has been used for detection of the CCHFV genome. RESULTS: This genome was detected in 19.2% of the ticks collected from livestock of different regions of the Hamadan province in western Iran. The infected species belonged to Hyalomma detritum, H. anatolicum, Rhipicephalus sanguineus and Argas reflexus. With one exception, genetic analysis of the virus genome isolates showed high sequence identity to each other. Even though they clustered in the same group with the strain circulating in Iran, they had a closer relationship to the Matin strain. INTERPRETATION & CONCLUSION: Vector control programs should be applied for reducing population density of potential tick vectors in this province. Further surveys are indicated in this region to provide a better view of the distribution and epidemiology of the virus.

Phylogenetic analysis in a recent controlled outbreak of Crimean-Congo haemorrhagic fever in the south of Iran, December 2008.

Crimean-Congo haemorrhagic fever (CCHF) is a viral zoonotic disease with a high mortality rate in humans. The CCHF virus is transmitted to humans through the bite of Ixodid ticks or contact with blood or tissues of CCHF patients or infected livestock. In December 2008, a re-emerging outbreak of CCHF occurred in the southern part of Iran. Five people were hospitalised with sudden fever and haemorrhaging, and CCHF was confirmed by RT-PCR and serological assays. One of the cases had a fulminant course and died. Livestock was identified as the source of infection; all animals in the incriminated herd were serologically analysed and more than half of them were positive for CCHFV. We demonstrated that two routes of transmission played a role in this outbreak: contact with tissue and blood of infected livestock, and nosocomial transmission. Phylogenetic analyses helped to identify the origin of this transmission. This outbreak should be considered as a warning for the national CCHF surveillance system to avoid further outbreaks through robust prevention and control programmes.

Risk of colorectal cancer in relatives: a case control study.

BACKGROUND: In around 10 to 15% of all colorectal cancer (CRC) cases, a positive family history of colorectal cancer is observed . Although increased risk of colorectal cancer in individuals with a family history of the disease has been observed consistently in the past studies, limited information is available on colorectal cancer associated with family history in Iran. AIMS: The purpose of this study is to define the risk of colorectal cancer associated with a family history of cancer. SETTING AND DESIGN: The present study was designed as an unmatched case control study. The cases were 393 patients with histologically confirmed colorectal carcinomas and there were 393 controls, randomly selected from among the healthy participants in a health survey. METHODS: The family history was extracted from a standard history form completed by the patient or from the record created by a health care provider. STATISTICAL ANALYSIS: Mantel-Heanszel Odds Ratio was computed for removing the confounding effect of age and sex. RESULTS: A positive family history of cancer was reported by 36.4 and 24.4% among the cases and controls, respectively. Colorectal cancer risk increased two-fold in subjects who reported having first degree relatives with cancer. The adjusted odds ratio was 4.76, indicating that having a positive family history of colorectal cancer among relatives increased one's risk of colorectal cancer about 4.5-fold. CONCLUSION: According to our findings, a family history of cancer increased the risk of CRC. Due to this fact that there is no current colorectal cancer screening program in Iran, it is recommended that first degree relatives of patients with colorectal cancer should be considered as a priority group for screening programs.

Characteristics of colorectal mucinous adenocarcinoma in Iran.

AIMS AND BACKGROUND: Mucinous adenocarcinoma (MA) colorectal cancer accounts for 10 to 15% of colorectal carcinoma. It is generally thought that patients with MA present at a more advanced stage of disease and have a poorer prognosis than those with other types of carcinoma. The purpose of the present study was to clarify the clinicopathological characteristics of mucinous colorectal carcinoma in the Iranian population. METHODS: Between January 2002 and March 2008, Of the 1283 colorectal cancer patients, 110 patients were considered to have mucinous tumors according to pathology report. Patients evaluated on the basis of sex, age, location of tumor, stage, differentiation of tumor and family history of cancer. Kaplan-Meier curves and log-rank tests were used for survival analysis. RESULTS: The median age of these patients at diagnosis was 50.07 years. More than 50% of patients were younger than aged 50 years. 34.5% of patients had a family history of colorectal cancer in their first-degree relatives. Most tumors were presented in right colon. 54.3% of MA patients had advanced stage lesions. The Kaplan-Meier method indicated that, the 1, 3 and 5 year survival rates are 92.6, 80.1 and 41.3 percent, respectively. Survival of the patients was related to disease stage (P = 0.023). CONCLUSION: Our suggests that genetic factors may be play an important role in the development of this disease in our country and screening programs, especially genetic screening programs, should be considered as a main measure for prevention and control of colorectal cancer in Iran.

Crimean-Congo hemorrhagic fever in Iran and neighboring countries.

Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic viral disease that is asymptomatic in infected livestock, but a serious threat to humans. Human infections begin with nonspecific febrile symptoms, but progress to a serious hemorrhagic syndrome with a case fatality rate of 2-50%. Although the causative virus is often transmitted by ticks, livestock-to-human and human-to-human transmissions also occur. The disease is one of the most widely distributed viral hemorrhagic fevers occurring in Africa, the Middle East, Asia, and some parts of Europe. In this study, we have focused on the CCHF situation in Iran and neighboring countries and provide evidence of over 5000 confirmed cases of CCHF in a single period/season.

Survival in gastric cancer patients: univariate and multivariate analysis.

BACKGROUND AND AIM: Cancer for a long time has been recognized as a fatal disease. Thus it is known to be major health problem in many countries throughout the world. In recent years, cancer morbidity and mortality increased in our country and especially gastric cancer has second order among all cancers. The aim of this study was to analysis the survival of patients diagnosed with gastric cancer and the factors which modify prognosis. METHODS: Retrospective study of overall patients diagnosed with gastric cancer registered in the cancer registry center of Research Center for Gastroenterology and Liver Disease (RCGLD), Shahid Beheshti University, M.C, Tehran, Iran, between Dec. 2001 and Dec. 2006 was done. Survival status of patients was followed by the telephone contact. The Kaplan-Meier statistical method was employed to determine the probability of survival and log-rank test to compare those. Cox regression was used to determine prognosis factors. P < 0.05 was considered as statistically significant. All calculations were carried out by SPSS (version13.0) statistical software. RESULTS: Of 746 patients, 38.6% are dead. The mean and median survival time was 42.45 and 22.8 months, respectively and five-year survival rate was 25.3%. With univariate analysis, Age at Diagnosis, surgery treatment, type of first treatment, pathologic stage, tumor size, histology type of tumor, extent of wall penetration and pathologic distant metastasis were significant prognostic factors related to overall survival time. Tumor size greater than 35mm (HR = 2.12) and have a metastasis (HR = 2.04) were found to be the statistically significant poor prognostic factors related to survival in multivariate analysis. CONCLUSIONS: According to results, early detection of cancer in lower ages and in primary grades of tumor is important to increase patient's life expectancy.

Surveillance and laboratory detection system of Crimean-Congo haemorrhagic fever in Iran.

Crimean-Congo haemorrhagic fever (CCHF) is a viral zoonotic disease with a high mortality rate in humans. The CCHF virus (CCHFV) is transmitted to humans through the bite of Ixodid ticks or by contact with blood or tissues of infected livestock. In addition to zoonotic transmission, CCHFV can be spread from person to person and is one of the rare haemorrhagic fever viruses able to cause nosocomial outbreaks in hospitals. Crimean-Congo haemorrhagic fever is a public health problem in many regions of the world such as Eastern Europe, Asia, the Middle East and Africa. In addition to clinical symptoms, the diagnosis of CCHF is based on the use of serological tests for the detection of immunoglobulin M and immunoglobulin G antibodies and on the use of molecular tools such as RT-PCR. From 1970 to 1978, serological and epidemiological studies were performed in humans and in livestock of Iran. After two decades and observations of CCHF in some provinces of Iran, a CCHF surveillance and detection system was established in 1999, leading to a dramatically decreased mortality rate from 20% (year 2000) to 2% (year 2007).

Colorectal cancer in Iran: an epidemiological study.

BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers worldwide. But information regarding CRC in Iran is limited; the aim of this study was to investigate the epidemiologic features of CRC in Iran, using 5-year data from cancer registry. METHODS: This survey is a descriptive-analytic study consists of 1138 colorectal cancer patients who registered in Cancer Registry Center of Research Center for Gastroenterology and Liver Disease, Tehran, Iran. These data gathered using interview and pathology reports that registered in cancer registry forms. Data analysis was performed with descriptive and univariate methods. RESULTS: Of 1138 patients, 696 cases were male and 442 female. There was no significant difference between males and females regarding age at diagnosis. 400 patients (35.1%) had a family history of cancer. The most common histology type of tumor was adenocarcinoma, NOS. In most cases (39.1%) tumor grading was well differentiated and there was no significant difference between males and females. CONCLUSION: Colorectal cancer is a disease with nonspecific symptoms. Family history of cancer was evident in 35.1% of our cases and also 42.9% of patients were below the age of 50 years old, suggests that genetic factors may be play an important role in the development of this disease in our country.